Meyerozyma guilliermondii

Synonymy:
Candida guilliermondii.

Meyerozyma guilliermondii has been isolated from numerous human infections, mostly of cutaneous origin.

It is also found on normal skin and in sea water, faeces of animals, fig wasps, buttermilk, leather, fish and beer.

RG-1 organism.

Culture:
White to cream-coloured smooth, glabrous, yeast-like colonies.

Microscopy:
Spherical to subspherical budding yeast-like cells or blastoconidia, 2.0-4.0 x 3.0-6.5 µm.

India ink preparation:
Negative - no capsules present.

Dalmau plate culture:
Branched pseudohyphae with dense verticils of blastoconidia.

Molecular identification:
ITS sequencing recommended.

MALDI-TOF MS:
Able to accurately identify this species.

Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data
Germ Tube	-	L-Sorbose	v	L-Arabinose	v	D-Glucitol	v
Fermentation		Sucrose	+	D-Arabinose	v	𝝰-M-D-Glucoside	v
Glucose	+	Maltose	+	D-Ribose	+	D-Gluconate	v
Galactose	v	Cellobiose	v	L-Rhamnose	v	DL-Lactate	v
Sucrose	+	Trehalose	+	D-Glucosamime	+	myo-Inositol	-
Maltose	-	Lactose	-	N-A-D-glucosamine	+	2-K-D-Gluconate	+
Lactose	-	Melibiose	v	Glycerol	+	D-Glucuronate	nd
Trehalose	+	Raffinose	+	Erythritol	-	Nitrate	-
Assimilation		Melezitose	v	Ribitol	+	Urease	-
Glucose	+	Soluble Starch	-	Galactitol	v	0.1% Cycloheximide	v
Galactose	+	D-Xylose	+	D-Mannitol	v	Growth at 37C	v

Key features:
Germ tube negative yeast and sugar assimilation pattern.

Antifungal susceptibility: *Meyerozyma guilliermondii* (Diekema *et al.* 2009; Pfaller *et al*., 2013a and Australian national data); **MIC µg/mL.**
Antifungal	No	≤0.016	0.03	0.06	0.125	0.25	0.5	1	2	4	8	16	32	≥64
AMB	243	2	2	9	72	80	53	16	2	1	6
FLU	244					1	7	17	96	83	21	9	3	7
ISAV	33		1	2	8	6	10	1	3	2
VORI	250	14	28	107	70	17	3	2	3			6
POSA	247	1	12	19	58	94	48	5	4			6
ITRA	69				11	16	35	5	1			1
ANID	172	1		4	8	6	16	69	59	8	1
MICA	162	3	1	4	11	23	68	37	13		1	1
5FC	67		8	54	2	1	1	1