Bromocresol Purple Milk Solids Glucose Agar (BCP).

For the differentiation of Trichophyton species (Fischer and Kane 1971; Summerbell et al. 1988).

Solution A

Distilled water 1000 mL

Skim milk powder (Carnation brand) 80 g

Bromcresol (or bromocresol) purple (1.6% solution in alcohol) 2 mL

Dissolve in 2 litre flask and autoclave 121C for 10 minutes.

Solution B

Glucose 40 g

Distilled water 200 mL

Dissolve and autoclave at 121C for 10 minutes.

Solution C

Bacto agar (BD 214010) 30 g

Distilled water 800 mL

Soak for 15 minutes in 3 litre flask; autoclave at 121C for 10 minutes.

Final mixture

To make media add solution A and B to solution C. Adjust final pH to 6.6.

Aseptically dispense for slopes (7 mL amounts into bottles).