Malt Extract Agar

For the routine cultivation and identification of fungi.

Ingredients

Malt extract (Oxoid L39) 20 g

Bacto agar (BD) 20 g

Distilled water 1000 ml

Method

Dissolve malt extract in a plastic beaker and pH the solution to pH 6.5 with NaOH.

Soak agar in small quantity of solution. Bring remaining solution to the boil, stirring constantly.

Add to soaking agar. Bring to boil, stirring constantly.

Dispense for slopes as required.

Autoclave at 121C for 10 minutes, remove and slope or pour for plates as required.