Sidelobe Suppressed Bessel Beams for Light Sheet Microscopy

Posted on Oct 7 2024 by a1612973

Imaging cell nuclei of the zebrafish eye using Bessel beam and Sidelobe Suppressed Bessel beam. Scale bar: 50 micron

The team at the University of St Andrews Optical Manipulation Group together with the Centre of Light for Life have published a new article on using sidelobe suppressed Bessel beams for light sheet microscopy:

Chetna Taneja, Jerin Geogy George, Stella Corsetti, Philip Wijesinghe, Graham D. Bruce, Maarten F. Zwart, Shanti Bhattacharya, and Kishan Dholakia, "Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy," Biomed. Opt. Express 15, 6183-6197 (2024)

Read article here: https://opg.optica.org/boe/fulltext.cfm?uri=boe-15-11-6183&id=561140###

The image, adapted from the paper, shows the difference for imaging GCaMP labelled cell nuclei of the zebrafish eye using (a) the Bessel beam (BB) vs (b) the sidelobe suppressed Bessel beam (SSBB).

Tagged in bessel beam, light sheet, microscopy, kishan dholakia